Identifying regulators of parental imprinting by CRISPR/Cas9 screening in haploid human embryonic stem cells

ABSTRACT: To search for factors regulating paternally imprinted genes (PEGs), we performed a genome-wide loss-of-function CRISPR/Cas9 screen in haploid parthenogenetic ESCs. This by staining a pooled CRISPR library with a PEG10 antibody and next FACS-sorted for cells that presented de-novo PEG10 expression.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Homo sapiens

SUBMITTER: Elyad Lezmi 

PROVIDER: E-MTAB-11014 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress