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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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PolII Rpb3 ChIP-seq data from D. melanogaster

ABSTRACT: We performed Pol II ChIP-seq experiments with 3rd instar larva salivary glands material from male, female and MSL2 RNAi D.melanogaster, as well as from S2 cell lines material. The antibody used recognizes the Rpb3 subunit of RNA Pol II irrespective of the phosphorylation state of the Rpb1 carboxy-terminal domain, therefore capturing Pol II during all stages of transcription. Corrected processed data has been made available following Vaquerizas et al (2013). [M-^SResponse to Comments on M-^SDrosophila Dosage Compensation Involves Enhanced Pol II Recruitment to Male X-Linked PromotersM-^T, Juan M. Vaquerizas, Florence M. G. Cavalli, Thomas Conrad, Asifa Akhtar, Nicholas M. Luscombe, Science 340 (6130): 273 (2013)]

INSTRUMENT(S): Illumina Genome Analyzer II

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Florence Cavalli

PROVIDER: E-MTAB-1112 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Response to Comments on "Drosophila Dosage Compensation Involves Enhanced Pol II Recruitment to Male X-Linked Promoters".

Vaquerizas Juan M JM Cavalli Florence M G FM Conrad Thomas T Akhtar Asifa A Luscombe Nicholas M NM

Science (New York, N.Y.) 20130401 6130

Ferrari et al. and Straub and Becker wrongly claim that an error in the computational analysis calls into question the conclusions of Conrad et al. All the available evidence, including the reanalyzed genomic data, show that the conclusions and the key message of the study remain unchanged: RNA polymerase II recruitment to male X-linked promoters is an important regulatory step during dosage compensation. ...[more]

PMID: 23599465

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