Transcription profiling of hESCs cultured on cSAPs and flat control (TCPS) using RNAseq analysis

ABSTRACT: The biophysical cues in the fate determination of pluripotent stem cells (PSCs) have not been well-revealed. In the current study, we used a novel type of substrate called colloidal self-assembled patterns (cSAPs) as an artificial matrix for the expansion of human ESCs. Briefly, the cSAPs substrate was composed of SiO2 with 2 μm diameter and PS with 100 nm diameter within the 24-well tissue culture plates (TCPS, Falcon). H1 hESCs cultured on cSAPs and flat control (TCPS) for 2 passages (4 days/passage) were dissociated with 0.5 mM EDTA for RNAseq. Total RNA (each sample ~ 1 × 106 cells) was extracted by 1 ml TRIzol (Life Technologies) and purified according to the standard instruction. RNA Sequence analysis was conducted by BGI Tech (Shenzhen, China). The analysis of gene function was perf

INSTRUMENT(S): BGISEQ-500

ORGANISM(S): Homo sapiens

SUBMITTER: Javad Harati 

PROVIDER: E-MTAB-11123 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress