Response of Leptospirillum ferriphilum DSM14647 to elevated NaCl concentration

ABSTRACT: Leptospirillum ferriphilum is an important acidophilic ferrous iron-oxidizing species for bioleaching or biooxidation to win metals like copper or gold, respectively. L. ferriphilum is inhibited by elevated conentrations of chloride. In the present study, the type strain of L. ferriphilum (i.e. strain DSM 14647) by subcultivation in presence of increasing chloride concentrations was adapted to tolerate higher concentrations of NaCl. The adapted culture was grown in presence of 180 mM NaCl or and the non-adapted culture without NaCl, and both were harvested in the late exponential phase. Total RNA was isolated and checked for quality and integrity. For each of the two conditions three RNA preparations of high quality (integrity number above 7) were pooled. Ribosomal RNA was depleted. DNA li

INSTRUMENT(S): Illumina MiSeq

ORGANISM(S): Leptospirillum ferriphilum

SUBMITTER: Michael Schlömann 

PROVIDER: E-MTAB-11136 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress