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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Bulk RNA sequencing of cycling and non-cycling NEUROG3-positive and negative human embryonic stem cell-derived pancreatic cells using a dual NEUROG3-reporter

ABSTRACT: Human embryonic stem cells with one allele of the NEUROG3 gene genetically modified into a fusion gene NEUROG3-LINKER-TAGRFPT-P2A-EGFP-NLS were differentiated into the pancreatic lineage until Stage 4 Day 1 of the in vitro differentiation protocol (Rezania et al., 2014, as modified in Petersen et al., 2017). GFP positive and negative cells were FAC-sorted according to their cell cycle state (G0/G1 or G2M), and the global transcriptomes of these four groups were compared using bulk RNA-seq.

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Homo sapiens

SUBMITTER: Belin Selcen Beydag-Tasöz

PROVIDER: E-MTAB-11210 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Single-cell RNA sequencing of human embryonic stem cell-derived pancreatic cells enriched for early endocrine progenitors using a novel dual NEUROG3-reporter

Project description:Human embryonic stem cells with one allele of the NEUROG3 gene genetically modified into a fusion gene NEUROG3-LINKER-TAGRFPT-P2A-EGFP-NLS were differentiated into the pancreatic lineage until Stage 4 Day 1 of the in vitro differentiation protocol (Rezania et al., 2014, as modified in Petersen et al., 2017). The cells were index-sorted according to their GFP expression to enrich for low GFP and high GFP cells, and deep single-cell RNA-seq was performed using the plate-based Smart-seq2 platform (Picelli et al. 2014).

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