Single cell analysis of CD45-positive immune cells infiltrating the central nervous system of mice undergoing experimental autoimmune encephalomyelitis.

ABSTRACT: C56BL/6 mice (10 to 12-week-old) were immunized subcutaneously at the base of the tail with 100 μg of MOG (35–55) emulsified in complete Freund's adjuvant containing 500 μg of Mycobacterium tuberculosis. Pertussis toxin (200 ng; List Biological Laboratories) was injected intravenously at days 0 and 2 post-immunization. At day 17 post immunization, central nervous system CD45-positive immune cells were isolated and loaded onto a single channel of a 10x Chromium chip for each sample.

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Mus musculus

SUBMITTER: Mohamed EL BEHI 

PROVIDER: E-MTAB-11294 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress