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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Donor-to-donor variation in gene expression profile of 3D bronchial epithelial cells after cigarette smoke exposure

ABSTRACT: Three-dimensional (3D) cultured primary cells are used to predict the toxicity of substances towards humans because these 3D cultures closely mimic the physiological architecture of tissues. Nonetheless, it is important to consider primary-cell-specific variability for endpoint selection and appropriate evaluation of toxicity because donor-dependent characteristics may be retained even in in vitro cell cultures. In this experiment, 3D differentiated bronchial epithelial cells from three donors were used to investigate donor-to-donor variability, with an aqueous extract of cigarette smoke used as the test substance. Therefore, global gene expression analysis was performed to expand and deepen our insight into the variability.

ORGANISM(S): Homo sapiens

SUBMITTER: Sakura Mori

PROVIDER: E-MTAB-11316 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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