MicroRNA profiling by array of human osteosarcoma and their normal bone counterpart

ABSTRACT: Profiling array: in order to investigate the microRNAs differentially expressed between osteosarcoma and their normal bone counterpart. Specimens were kept at 4 C in RNAlater for up to 1 week, then stored at 80 C. When formal pathologic interpretation of histology from other portions of the biopsy specimen rendered a diagnosis of osteosarcoma, the RNA-preserving tissue specimens were banked and annotated. In preparation for these specific experiments, total RNA was extracted from banked specimens with the TRIzol reagent and method (Invitrogen). Control samples were derived from to-be-discarded bone fragments obtained from similarly consented patients undergoing debridement surgeries for acute, traumatic injuries to the long bones.

ORGANISM(S): Homo sapiens

DISEASE(S): osteosarcoma

SUBMITTER: Marco Galasso 

PROVIDER: E-MTAB-1136 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress