RNA-seq of the RNA cargo of extracellular vesicles released in perfusate and bile during ex situ isolated liver perfusion of porcine livers exposed to 60-minutes warm ischemia vs. control (no warm ischemia)

ABSTRACT: There is a ever widening gap between the availability of donor livers suitable for transplantation and liver transplant demand of a growing waiting list. To expand the pool of liver grafts, donation after circulatory death is increasingly being used. Livers procured after circulatory death are exposed to a harmful period of warm Ischemia (WI) during the donation process, reducing post-transplant results significantly. Additionally, the conventional technique of static cold storage does not protect grafts that suffered WI. New dynamic preservation strategies by means of Normothermic Machine Perfusion (NMP) showed enhanced preservation of these type of grafts, but it is unknown how NMP influences liver cell biology during perfusion. Hepatocytes and cholnagiocytes have been shown to release E

INSTRUMENT(S): Illumina HiSeq 4000

ORGANISM(S): Sus scrofa

SUBMITTER: Nicholas Gilbo 

PROVIDER: E-MTAB-11420 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress