10x single cell RNAseq of chick ectoderm from stages HH5 to ss4

ABSTRACT: The vertebrate ectoderm gives rise to a variety of cell lineages, including neural, neural crest, placodal and non-neural cell fates. How cell fates are specified at the neural plate border (the region surrounding the neural plate) is not fully understood. We therefore carried out 10x scRNAseq of the chick epiblast to investigate cell fate specification at the neural plate border. Embryos were dissected and pooled according to stage. The tissue was then dissociated and FAC sorted to remove dead cells and remaining doublets before cells were stored in MeOH. Due to the time required to dissect embryos, multiple rounds of collections were carried out, with collections from the same stage pooled prior to 10x sequencing. Libraries were sequenced using an Illumina HiSeq 4000 at the Francis Crick

INSTRUMENT(S): Illumina HiSeq 4000, 10x 3’ single-cell mRNA v3 kit (10x Genomics)

ORGANISM(S): Gallus gallus

SUBMITTER:  

PROVIDER: E-MTAB-11444 | biostudies-arrayexpress |

SECONDARY ACCESSION(S): E-MTAB-10408

REPOSITORIES: biostudies-arrayexpress