Optimizing scalable full-transcript coverage single cell RNA sequencing using Smart-seq3xpress

ABSTRACT: Smart-seq3xpress was carefully optimized and >1,000 conditions were evaluated. This data submission is organized in 15 datasets that each contain fastq files, unmapped bam files, read count tables, UMI count tables and a barcode annotation file. The barcode_annotation.txt files contain the exact factors/variables tested. Below a short description of each set of experiments: K562_lowvolume: Evaluation of scaling volumes of Smart-seq3 (indicated volume refers to total volume in PCR), whether overlay was used and if cDNA was bead-cleaned or diluted prior to tagmentation. Cell input was K562 cells. The columns \\"treatment\\", \\"volume\\" and \\"VL\\" indicate the experimental parameters. HEK_lowvolume: Evaluation of scaling volumes of Smart-seq3 (indicated volume refers to total volume in

INSTRUMENT(S): DNBSEQ-G400, NextSeq 500

ORGANISM(S): Homo sapiens

SUBMITTER: Christoph Ziegenhain 

PROVIDER: E-MTAB-11467 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress