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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Integration of next-generation sequencing and proteomics data: application to the discovery of new coding events in the human spermatozoa

ABSTRACT: Proteomics and transcriptomics data were integrated in order to identify hard-to-reach uncharacterized proteins which are known to be particularly numerous in human spermatozoa. MS-identifications were launched both against UniprotKB and translated predicted ORF databases generated from four different RNAseq processing workflows. For each validated peptide-spectrum match (PSM), not identified in UniprotKB, but at least in one of the four customized databases, spectrum was carefully checked manually to assess the novel identification reliability.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Homo sapiens

SUBMITTER: Aubry Marc

PROVIDER: E-MTAB-11518 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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