High resolution chemical-genomics identifies targets for synergistic antibiotics with aztreonam

ABSTRACT: A whole genome screen was used to assay every gene of Escherichia coli strain BW25113 to identify genes involved in susceptibility to the monobactam (beta-lactam) antibiotic aztreonam. The methodology has been called TraDIS-Xpress, and is a version of TraDIS or Tn-seq. A transposon mutant library consisting of several hundred thousand mutants was constructed using a Tn5-derived transposon incorporating an inducible outward transcribing promoter. All the mutants were grown in LB broth cultures supplemented with aztreonam at 2 x, 1 x, 0.5 x and 0.25 x MIC with induction of the transposon promoter using 0.2 mM IPTG or 1 mM IPTG or without induction. Following growth, mutants with increased susceptibility show reduced numbers and those with reduced susceptibility show increased numbers. Each

INSTRUMENT(S): NextSeq 550

ORGANISM(S): Escherichia coli str. K-12 substr. MG1655

SUBMITTER: Arthur Turner 

PROVIDER: E-MTAB-11576 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress