Single-cell RNA-Sequencing of testis tissue from 3 mouse species and their immediate crosses

ABSTRACT: We performed an inter-species comparison of murine spermatogenesis using the inbred strains C57B6J, CAST/EiJ and CAROLI/EiJ to investigate the cell type-specific evolution of gene expression levels among closely related species. We also used F1 crosses of C57B6J and CAST/EiJ to investigate context-specific regulatory effects on gene expression in cis and trans by measuring allele-specific expression (Goncalves et al. 2012; Wittkopp et al. 2021). To this end, single-cell RNA-Sequencing data was generated from dissociated testicular tissue in each mouse strain using the 10x Genomics scRNA-Seq platform.

INSTRUMENT(S): Illumina HiSeq 2500, Illumina NovaSeq 6000

ORGANISM(S): Mus musculus

SUBMITTER:  

PROVIDER: E-MTAB-11602 | biostudies-arrayexpress |

SECONDARY ACCESSION(S): E-MTAB-6934

REPOSITORIES: biostudies-arrayexpress