In-Vitro Cell Adhesion Assay

ABSTRACT: The adhesion of monocytic cells to the M-^SdysfunctionalM-^T endothelium constitutes a critical step in the initiation of atherosclerotic plaque formation. Cigarette smoke (CS) has been shown to contribute to the monocyte-endothelial adhesion process. Yet, the complex mechanisms underlying this event remain to be discovered. In the systemic compartment, monocytes and endothelial cells are exposed primarily to soluble constituents originating from the inhaled CS and absorbed through the lung alveolar epithelium and the adjacent microvascular endothelium. Considering this rationale, we developed an in vitro adhesion assay, intending to mimic the in vivo situation, to deeply investigate the impact of CS on the adhesion of monocytic cell to the endothelium. Using a transcriptomics approach fol

INSTRUMENT(S): Affymetrix GeneChip Scanner 3000 7G

ORGANISM(S): Homo sapiens

SUBMITTER: Sam Ansari 

PROVIDER: E-MTAB-1173 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress