Microarray of Ruminiclostridium cellulolyticum parental strain and mutants grown on cellulose

ABSTRACT: R. cellulolyticum strains (the parent strain, ΔccpA, ΔccpB and Δcph) were cultivated in defined VM medium with cellulose (10 g/L).Since ΔccpA and Δcph could not grow on cellulose, we performed an incubation experiment to understand how ΔccpA and Δcph respond to cellulose. Specifically, the parent strain, ΔccpA and Δcph were grown to an OD600 of 0.5-0.6 in 50 ml defined VM media with cellobiose as the carbon source. Each strain had three biological replicates. Bacterial cells from each biological replicate were then collected by centrifugation at 4000 g and washed twice with the defined VM medium (no carbon added). Finally, washed cells from each biological replicate were inoculated into the defined VM medium with 10 g/L cellulose. During shaking incubation at 34°C, samples were collected a

ORGANISM(S): Ruminiclostridium cellulolyticum H10

SUBMITTER: Xuanyu Tao 

PROVIDER: E-MTAB-11774 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress