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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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RNA-seq of mouse intestinal organoids treated with TGF-beta

ABSTRACT: We aimed to investigate gene expression changes in intestinal organoids from different mouse genotypes after treatment with TGF-beta. Wild-type, villinCreER;KrasG12D/+;Trp53fl/flRosa26N1icd/+ (KPN), and villinCreER;Apcfl/fl;KrasG12D/+;Trp53fl/flTgfbrIfl/fl (AKPT) intestinal organoids were plated, and the media was supplemented with 5ng/mL of recombinant mouse TGFß1 protein on Day 3. RNA was collected 24h later and processed for RNA sequencing.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Mus musculus

SUBMITTER: Simon Leedham

PROVIDER: E-MTAB-11784 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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