ChIP-seq identification of RbpA (MSMEG_3858) binding sites in Mycobacterium smegmatis
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ABSTRACT: RbpA is an essential transcription factors in mycobacteria. For ChIP-seq, we used Mycobacterium smegmatis strains with RbpA-FLAG (LK2541), where an additional copy of RbpA under anhydrotetracycline inducible promoter was stably inserted into the genome. RbpA-FLAG was expressed in exponential phase and ChIP performed at mid-exponential phase of growth.
INSTRUMENT(S): NextSeq 550
ORGANISM(S): Mycolicibacterium smegmatis MC2 155
SUBMITTER:
PROVIDER: E-MTAB-11829 | biostudies-arrayexpress |
SECONDARY ACCESSION(S): E-MTAB-7004
REPOSITORIES: biostudies-arrayexpress
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