Gene expression profiles of the EBV-induced lymphomas against ‘normal’ human PBL

ABSTRACT: Peripheral blood lymphocytes (PBLs) were isolated from the fresh blood samples of six healthy donors and intraperitoneally grafted into SCID mice at 8~10 × 107 PBLs/mouse. PBLs from each donor were grafted into 3~4 SCID mice. EBV was isolated from the supernatant of B95-8 cells cultured in vitro under starvation conditions. EBV infection was not required for SCID mice that were grafted with PBLs from donors who were positive for EBV viral capsid antigen (VCA)-IgG and IgA. On the other hand, 0.4 mL of EBV suspension was intraperitoneally injected into SCID mice that were grafted with PBLs from donors who were VCA-IgA negative on day 3 post-grafting. All surviving mice were euthanized on day 135 post-PBL grafting. Total RNA was extracted from the six EBV-induced lymphomas (tumor group) and n

ORGANISM(S): Homo sapiens

SUBMITTER: Chengkun Wang 

PROVIDER: E-MTAB-11857 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress