Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

13 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ScRNA-seq of lung CD140a+ mesenchymal cells from naïve or tumor-bearing CD140aEGFP reporter mice

ABSTRACT: To investigate the CD140a+ mesenchymal cell heterogeneity in the lung in both steady and tumor-bearing conditions, we utilized CD140aEGFP reporter mice, and purified CD140a+ lung mesenchymal cells from naive and tumor-bearing mice and performed scRNA-seq.

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Mus musculus

SUBMITTER: Jiayuan Shi

PROVIDER: E-MTAB-11902 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

scRNA-seq of lung CD140a+ mesenchymal cells from AT3-gcsf tumor-bearing mice

Project description:To characterize lung CD140a+ mesenchymal cells in host neutrophilic inflammation conditions, we isolated lung CD140a+ mesenchymal cells from AT3-gcsf tumor-bearing CD140a-EGFP mice for scRNA-seq.

2023-02-01 | E-MTAB-12371 | biostudies-arrayexpress

scRNA-seq of lung CD140a+ mesenchymal cells (fibroblasts) from CD140a-EGFP reporter mice

Project description:To investigate the heterogeneity of lung CD140a+ mesenchymal cells (fibroblasts) and identify the specific lung fibroblast subset, we purified CD140a+ lung cells using CD140a-EGFP reporter mice for a higher-resolution scRNA-seq.

2022-07-19 | E-MTAB-11875 | biostudies-arrayexpress

RNA-seq of ex vivo cultured lung CD140a+ mesenchymal cells (fibroblasts) upon stimulation by IL-1β

Project description:To gain a better understanding of the role of Interleukin-1β (IL-1β) in lung CD140a+ mesenchymal cells (fibroblasts) modulation, we performed RNA-seq to compare the transcriptomes of IL-1β-treated and control lung CD140a+ mesenchymal cells (fibroblasts).

2022-07-19 | E-MTAB-11887 | biostudies-arrayexpress

RNA-seq of BM neutrophils after cultured with lung CD140a+ MCs

Project description:To determine how neutrophils are reprogrammed by lung CD140a+ MCs (mesenchymal cells), we employed lung CD140a+ MCs, and ex-vivo cultured with BM(bone marrow)-derived neutrophils. After the treatment, neutrophils were harvested for RNA extraction and the transcriptional profiles were analyzed by RNA sequencing (RNA-seq).

2023-02-01 | E-MTAB-12366 | biostudies-arrayexpress

CD140a+ human glial progenitor cells

Project description:Glial progenitor cells (GPCs) pervade the human brain. These cells express gangliosides recognized by MAb A2B5, and some but not all can generate oligodendrocytes. Since some A2B5+ GPCs express PDGFa receptor (PDGFRa), which is critical to oligodendrocyte development, we asked if PDGFRa-directed sorting might isolate oligodendrocyte-competent progenitors. We used FACS to sort PDGFRa+ cells from the second trimester fetal human forebrain, based on expression of the PDGFRa epitope CD140a. CD140a+ cells could be maintained as mitotic progenitors that could be instructed to either oligodendrocyte or astrocyte phenotype. Transplanted CD140a+ cells robustly myelinated the hypomyelinated shiverer mouse brain. Microarray confirmed that CD140a+ cells differentially expressed PDGFRA, NG2, OLIG1/2, NKX2.2 and SOX2. Some expressed CD9, thereby defining a CD140a+/CD9+ fraction of oligodendrocyte-biased progenitors. CD140a+ cells differentially expressed genes of the PTN-PTPRZ1, wnt, notch and BMP pathways, suggesting the interaction of self-renewal and fate-restricting pathways in these cells, while identifying targets for their mobilization and instruction. 10 samples, 5 CD140a+, and 5 CD140a- sorted samples for individual fetal human brain

2011-09-30 | E-GEOD-29368 | biostudies-arrayexpress

scRNA-seq of lung stromal cells (CD45-CD31-CD326-) from C57BL/6J mice

Project description:To investigate the heterogeneity of lung stromal cells and identify the specific lung stromal subset, we performed single cell RNA-sequencing (scRNA-seq) on lung stromal cells (CD45-CD31-CD326-). Around 6800 cells were captured using the 10x Chromium technology.

2022-07-19 | E-MTAB-11879 | biostudies-arrayexpress

CD140a+ human oligodendrocyte progenitor cells

2011-10-01 | GSE29368 | GEO

scRNA-seq of lung CD140a+ mesenchymal cells (fibroblasts) from CD140a-EGFP reporter mice

Project description:scRNA-seq of lung CD140a+ mesenchymal cells (fibroblasts) from CD140a-EGFP reporter mice

| PRJEB54002 | ENA

Cellular plasticity and diversity during heart valve epithelial-to-mesenchymal transitions revealed using single-cell RNA-seq

Project description:We have generated scRNA-seq data from embryonic day (E)10.5 and E12.5 atrioventricular canals (primitive heart valves) to assess cellular diversity during the distinct epithelial-to-mesenchymal transitions (EMTs) from endocardium and epicardium that guide the formation of valve mesenchyme. Alongside, wildtype atrioventricular canals, we generated scRNA-seq data from Sox9 conditional knockouts (Sox9fl/fl;Tie2-cre) to explore the role of SOX9 in EMT. Atrioventricular canals were microdissected from cardiac chambers and outflow tract, enriching for heart valve progenitor lineages.

2023-06-30 | E-MTAB-11795 | biostudies-arrayexpress

Transcription profile of fetal human CD140a-defined OPC differentiation in vitro

Project description:Human fetal dissociates from 19-22 week gestational age were magnetically sorted for CD140a antigen. CD140a-defined OPCs were plated into serum free conditions and allowed to differentiate in the absence of growth factors or mitogens. RNA was extracted from cells immediately following isolation and every day for 4 days. To block differentiation, matched cells were cultured in the presence of PDGF-AA (10ng/ml). This treatment prevents the acquisition of O4-positive oligodendrocyte cell fate and delays MBP mRNA expression by human CD140a-sorted OPCs. 29 samples, 4 time points, 2 media conditions, at least three biological replicates per time point and media condition

2016-07-01 | E-GEOD-36431 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data