Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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H3K27ac and H3K4me3 ChIP-Seq of livers from male mice fed a control or high-fat diet and treated with estrogen receptor agonists


ABSTRACT: Metabolic dysfunction-associated steatotic liver disease is the most prevalent liver disease and affects a quarter of the global population. Estrogens are associated to safeguard the liver from metabolic diseases. We fed male mice a control or high-fat diet for 13 weeks which induced fatty liver. We injected a subset of male mice fed a high-fat diet with four different estrogen receptor (ER) agonists for the last three weeks of the high-fat diet, activating ERalpha or ERbeta. Livers were collected and crosslinked using formaldehyde. ChIP-seq against H3K27ac (Abcam #4729, 5ug per IP) or H3K4me3 (Merck 05-1339, 5ug per IP) was performed, followed by library preparation and sequencing on an Illumina NextSeq 500 instrument. RNA-seq of the same individual mice was performed and is accessible under E-MTAB-11833. The mouse identification column ('Sample' followed by number) can be used to match the individual mice between the experiments.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Mus musculus

SUBMITTER:  

PROVIDER: E-MTAB-11929 | biostudies-arrayexpress |

SECONDARY ACCESSION(S): E-MTAB-11833

REPOSITORIES: biostudies-arrayexpress

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