Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

12 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Multiome experiment from GLI3 KO brain organoids

ABSTRACT: To study the effect of GLI3 knockout on early brain organoid development, we collected single-cell multiome data from 18 day old brain organoids

ORGANISM(S): Homo sapiens

SUBMITTER: Sophie Jansen

PROVIDER: E-MTAB-12002 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Multiome scRNA- and scATAC-seq for SHH treated brain organoids

Project description:To investigate how SHH treatment influences patterning of early brain organoids, we performed multiome sequencing of brain organoids during early development

2022-09-01 | E-MTAB-12003 | biostudies-arrayexpress

Cut&Tag of GLI3 binding during early organoid development

Project description:To investigate GLI3 TF targets during organoid fate decisions, we performed Cut&Tag of GLI3 binding

2022-08-28 | E-MTAB-12006 | biostudies-arrayexpress

scRNA-seq data from GLI3 KO in brain organoids

Project description:To study the effect of GLI3 knockout in brain organoids, we collected scRNA-seq data from 45 day old brain organoids with GLI3 KO

2022-08-19 | E-MTAB-11997 | biostudies-arrayexpress

Multiome experiment of the neuroepithelial stage of brain organoid developement

Project description:To investigate the heterogeneity during the neuroepithelial stage of organoid development, we performed a multiome experiment on day 15-18 old brain organoids

2022-10-03 | E-MTAB-12004 | biostudies-arrayexpress

scRNA-seq of early brain organoid development from pluripotency

Project description:To study developmental trajectories in brain organoids, we conducted scRNA-seq and scATAC-seq in parallel on a dense timecourse of early development.

2022-08-18 | E-MTAB-12001 | biostudies-arrayexpress

scATAC-seq of early brain organoid development from pluripotency

Project description:To study developmental trajectories in brain organoids, we conducted scRNA-seq and scATAC-seq in parallel on a dense timecourse of early development.

2022-08-18 | E-MTAB-11998 | biostudies-arrayexpress

Amplicon sequencing data from CROP-seq in 2 month old organoids

Project description:To better detect CROP-seq mRNAs in our CROP-seq experiment, we performed amplicon sequencing on the 10X single-cell cDNA libraries

2022-08-15 | E-MTAB-12005 | biostudies-arrayexpress

Combination of a microfluidic device with brain extracellular matrix promotes structural and functional maturation of human brain organoids

Project description:Brain organoids derived from human pluripotent stem cells provide a highly valuable in vitro model to recapitulate human brain development and neurological diseases. However, the current systems for brain organoid culture require further improvement for the reliable production of high-quality organoids. Here, we demonstrate two engineering elements to improve human brain organoid culture, (1) a human brain extracellular matrix (BEM) to provide brain-specific cues and (2) a microfluidic device with periodic flow to improve the survival and reduce the variability of organoids. A three-dimensional culture modified with BEM significantly enhanced neurogenesis in developing brain organoids from human induced pluripotent stem cells. Cortical layer development, volumetric augmentation, and electrophysiological function of human brain organoids were further improved in a reproducible manner by dynamic culture in microfluidic chamber devices. Our engineering concept of reconstituting brain-mimetic microenvironments facilitates the development of a reliable culture platform for brain organoids, enabling effective modeling and drug development for human brain diseases.

2021-06-02 | PXD025397 | Pride

Gli3 ChIP

Project description:Gli3 ChIP cortex

| PRJEB27543 | ENA

RNA-seq of brain organoids from human NKX2.1(GFP/w) hESCs treated with patterning molecules

Project description:There are on-going efforts to steer brain organoid development toward distinct regional identities by supplying cues in defined culture conditions. Here we incubated developing organoids with a number of patterning molecules at different concentrations to assess their effects on establishing brain region identities.

2021-02-11 | E-MTAB-10037 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data