Multi-angled transcriptome analyses reveal chameleon-like behavior of macrophages in the porcine respiratory tract

ABSTRACT: Macrophages have a pivotal role during viral infections in pigs. By expressing cell surface receptors, macrophages become viral targets and reservoirs. In the case of upper respiratory tract infections, many viruses target the peripheral nasal mucosa. Recent in vivo and in vitro (primary nasal cell cultures) porcine reproductive and respiratory syndrome virus (PRRSV) studies demonstrated that several macrophage subsets exist in the porcine nasal mucosa, and that virus strains with different virulence showed altered tropism for these different macrophage populations. To further investigate these macrophage subsets, total RNA sequencing was performed in parallel on two subsets from the nasal mucosa and lung macrophages. Macrophages were isolated by either fluorescent activated cell sorting (

INSTRUMENT(S): Illumina MiSeq

ORGANISM(S): Sus scrofa

SUBMITTER: Nick Vereecke 

PROVIDER: E-MTAB-12120 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress