De novo comparative transcriptome analyses of strawberry (Fragaria vesca) genotypes in response to Phytophthora cactorum.

ABSTRACT: In this RNA-seq study, we compared the transcriptome of three Fragaria vesca genotypes in response to Phytophthora cactorum. The goal of our study was to dissect the resistance mechanism of the diploid strawberry (F. vesca) that are resistant to P. cactorum. A susceptible genotype (NCGR1218) and two resistant (NCGR1603 and Bukammen) F. vesca genotypes were used for the comparative transcriptome analyses. Plants were inoculated with P. cactorum zoospores (2mL of 2 × 105 spores/mL) in the crown (rhizome) and sampled 48 hours later. The appropriate controls for each genotype were i) samples wounded and inoculated with water and sampled 48 hours after the treatment and ii) untreated samples. Four biological replicates, each consisting of four individual test plants from each genotype were used

INSTRUMENT(S): Illumina HiSeq 3000

ORGANISM(S): Fragaria vesca

SUBMITTER: Anupam Gogoi 

PROVIDER: E-MTAB-12152 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress