Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

13 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Whole genome sequencing of brefeldin A adaptors in Cryptococcus neoformans

ABSTRACT: Cryptococcus neoformans lab strain H99 was grown in YPD broth supplemented with 16ug/ml brefeldin A for 72h at 30C. We used 3 biological repeats. Then the culture was washed and diluted with distilled water, then plated on YPD plate. From each culture, we randomly tested 120 colonies to see if they obtained tolerance to brefeldin A. In total, we got 30 tolerant adaptors. We sequenced the adaptors as well as the parent.

INSTRUMENT(S): DNBSEQ-T7

ORGANISM(S): Cryptococcus neoformans

SUBMITTER: Sun Liuliu

PROVIDER: E-MTAB-12220 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Whole genome sequencing of adaptors selected by sub-inhibitory amount of brefeldin A in Candida albicans

Project description:In Candida albicans, the lab strain SC5314 was grown in YPD broth supplemented with 4ug/ml brefeldin A for 24h. The culture was washed, diluted and spread on YPD. Randomly 120 colonies were tested for tolerance to brefeldin A. Two adaptors (FY200 and FY201) obtained tolerance. These two adaptors were sequenced.

2023-10-06 | E-MTAB-12490 | biostudies-arrayexpress

Whole genome sequencing of Cryptococcus neoformans adaptors derived from exposure to supra-MIC amount of brefeldin A

Project description:Cryptococcus neoformans lab strain H99 was spread on YPD plates supplemented with 64ug/ml brefeldin A. Randomly 60 adaptors were chosen. These adaptors, as well as the parent were sequenced.

2022-10-31 | E-MTAB-12318 | biostudies-arrayexpress

Whole genome sequencing of Candida albicansSC5314 treated with brefeldin A

Project description:We treated Candida albicans lab strain SC5314 with brefeldin A and we did whole genome sequencing of 18 adaptors

2021-04-23 | E-MTAB-10351 | biostudies-arrayexpress

Whole genome sequencing of Chr3x3 derived brefeldin A adpators in Candida albicans

Project description:In Candida albicans, SC5314 is the wild type strain. FY1398 is Chr3 trisomy strain derived from SC5314. In this study, approximately 1 million cells of FY1398 were spread on YPD plate supplemented with 128 ug/ml brefeldin A. Randomly 18 adpators (LH40-LH57) were chosen. These adaptors were sequenced.

2023-09-07 | E-MTAB-12510 | biostudies-arrayexpress

Whole genome sequencing of VMA11 deletion strain derived fluconazole adaptors in Candida albicans

Project description:Candida albicans strains SC5314 and YJB-T1891 were used as wild type to delete VMA11. The deletion strains (TJ926 and TJ927) were spread on YPD plate supplemented with 1μg/ml fluconazole. Randomly 96 adaptors derived from TJ926 and 96 adaptors derived from TJ927 were tested with disk diffusion assay. Among the resistant adaptors, randomly 15 adaptors (A4-A18) and 48 adaptors (A19-A66) derived from TJ926 were sequenced.

2023-02-28 | E-MTAB-12673 | biostudies-arrayexpress

Whole genome sequencing of tebuconazole adaptors in Candida auris

Project description:Candida auris clinical isolate FY279 was exposed to tebuconazole (32μg/ml). Randomly14 adaptors were chosen. 10 adaptors obtained resistance to tebuconazole. These resistant adaptors were sequenced.

2023-05-10 | E-MTAB-12802 | biostudies-arrayexpress

Whole genome sequencing of caspofungin adaptors in Candida auris

Project description:Candida auris clade III isolate B12039 was spread on YPD plate supplemented with 250 ng/ml, 500 ng/ml, 1000 ng/ml, and 2000 ng/ml of caspofungin. In total, 33 adaptors were chosen for further analysis. We did sequencing of them as as well as the parent. 9 adaptors (250-1 - 250-9) were obtained from YPD+250ng/ml caspofugin. 6 adaptors (500-1 - 500-6) were obtained from YPD+250ng/ml caspofugin. 6 adaptors (1000-1 - 1000-6) were obtained from YPD+250ng/ml caspofugin. 12 adaptors (2000-1 - 2000-12) were obtained from YPD+250ng/ml caspofugin

2021-07-23 | E-MTAB-10627 | biostudies-arrayexpress

Whole genome sequencing of amphotericin B adaptors in Cryptocooccus neoformans

Project description:We exposed Cryptococcus neoformans lab strain H99 to amphtericin B and obtained some adaptors. We did sequencing of these adaptors.

2021-03-12 | E-MTAB-10172 | biostudies-arrayexpress

Whole genome sequencing of SC5314-derived fluconazole adaptors

Project description:Candida albicans lab strain SC53114 was used to obtain fluconazole-tolerant adaptors. 53 adaptors as well as the parent strain were sequenced.

2022-09-24 | E-MTAB-12175 | biostudies-arrayexpress

Whole genome sequencing of caspofungin adaptors derived from a chromosome 1 trisomy strain in Candida parapsilosis

Project description:Candida parapsilosis chromosome 1 trisomy strain TJ74 was exposed to 400ng/ml caspofungin. Randomly 30 adaptors were chosen. These adaptors were sequenced.

2022-09-30 | E-MTAB-12321 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data