TempO-Seq of human breast cancer MCF-7 and HCC1143 cell lines under chronic normoxia and hypoxia

ABSTRACT: Comparison of luminal and basal breast cancer cells under chronic normoxia and hypoxia. Cells were plated in 96-well plates and incubated 5 days under normoxia or hypoxia after which the wells were washed once with cold PBS and lysed using TempO-Seq lysis buffer for 15 min at room temperature. Samples were stored at −80 °C before shipping to BioClavis for whole genome TempO-Seq analysis. For normoxia (NX) 21% oxygen was used and for hypoxia (HX) 1%oxygen was used. Each condition has 3 biological replicates.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Homo sapiens

SUBMITTER: Sylvia Dévédec 

PROVIDER: E-MTAB-12233 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress