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Targeted nanopore resequencing and methylation analysis of LINE-1 retrotransposons

ABSTRACT: We used the nanopore Cas9 targeted sequencing (nCATS) strategy to specifically sequence 125 L1HS-containing loci in parallel and measure their DNA methylation levels using nanopore long-read sequencing. Each targeted locus is sequenced at high coverage (~45X) with unambiguously mapped reads spanning the entire L1 element, as well as its flanking sequences over several kilobases. The genome-wide profile of L1 methylation was also assessed by bs-ATLAS-seq in the same cell lines (E-MTAB-10895).

INSTRUMENT(S): MinION

ORGANISM(S): Homo sapiens

SUBMITTER: Gael Cristofari

PROVIDER: E-MTAB-12247 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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