Bulk RNA-seq of PSCs and induced PSM cells from six different mammalian species

ABSTRACT: In this study, we performed bulk RNA-seq of pluripotent stem cells (PSCs) and induced pre-somitic mesoderm (PSM) cells of six different mammalian species. The species studied are: mouse (Mus musculus), marmoset (Callithrix jacchus), rabbit (Oryctolagus cuniculus), human (Homo sapiens), cattle (Bos taurus) and southern white rhinoceros (Ceratotherium simum). We used mouse ESCs, marmoset iPSCs, rabbit ESCs, human iPSCs, bovine ESCs and rhinoceros ESCs to induce PSM-like cells from these species following protocols already described. PSC samples were extracted under maintenance conditions. PSM samples were extracted on the day when the differentiation efficiency was higher based on the percentage of cells expressing the PSM marker TBX6. We used identical culture conditions when extracting the

INSTRUMENT(S): Illumina NextSeq 500

ORGANISM(S): Oryctolagus cuniculus

SUBMITTER: Charles Girardot 

PROVIDER: E-MTAB-12263 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress