Transcriptome analysis of one aneuploid Cryptococcus neoformans strain
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ABSTRACT: Cryptococcus neoformans lab strain H99 was used to obtain tunicamycin adaptors. Transcriptome of one aneuploid adaptor, FY1381, was compared to parent.
INSTRUMENT(S): BGISEQ-500
ORGANISM(S): Cryptococcus neoformans var. grubii H99
Project description:Cryptococcus neoformans lab strain H99 was exposed to 600ng/ml aureobasidin A. After 3h, transcriptome was compared to no drug treatment control.
Project description:Cryptococcus neoformans lab strain H99 was used to obtain brefeldin A adaptors. Transcriptomes of 4 adaptors, each bearing a unique aneuploid, were compared to H99.
Project description:In Candida parapsilosis, transcriptomes of two aneuploid adaptors, TJ60 and TJ74, were compared to the euploid parent #12108. Cells were grown in YPD broth at 37C from OD600=0.1 to 1.0. Cells were collected and RNA was extracted.
Project description:Candida glabrata lab strain BG2 was used as parent to obtain some aneuploids by exposure to tunicamycin. Transcriptomes of 9 aneuploids bearing different chromosome change were compared to parent BG2
Project description:Two methods were used to induce petites from S, cerevisiae wild type strain MK288:.(1) MK288 was spread on YPD plate supplemented with 8 μg/ml fluconazole (2) MK288 was grown in YPD broth supplemented with 25 μg/ml ethidium bromide. Transcriptomes of the petites (TJ140 and TJ235) were compared to the wild type.
Project description:Candida albicans lab strain SC5314 was used as parent to obtain tebuconazole adaptors. Two adaptors, TJ1503 and TJ1669 were aneuploid. Transcriptomes of these two strains were compared to SC5314.
Project description:Candida auris wild type strain FY279 was used to tebuconazole adaptors. Transcriptomes of two adaptors, TJ42 and TJ44, were compared to wild type.
Project description:Candida albicans lab strain SC5314, which is a euploid diploid, was used to generate aneuploid fluconazole adaptors. Transcriptomes of aneuploids were compared to the euploid parent by growing cells in rich YPD broth.
Project description:Candia albicans strains SC5314, YJB-T490 and YJB-T1891 were grown in yeast extract peptone dextrose (YPD) broth at 30°C and 37°C, and exposed to 2 ug/ml and 16 ug/ml of fluconazole. 3h later, cells were harvested. Transcriptome was compared to cells grown in the absence of fluconazole.
Project description:Cryptococcus neoformans lab strain H99 was treated with 8ug/ml and 32ug/ml brefeldin A for 3h. The transcriptomes were compared to no drug treated cells.