Whole genome sequencing of supra-MIC tunicamycin adaptors in Cryptococcus neoformans
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ABSTRACT: Cryptococcus neoformans lab strain H99 was spread on YPD plate supplemented with 0.25ug/ml tunicamycin. Randomly 30 adaptors were chosen. 10 of them were used to obtain revertatants which lost aneuploidy step by step. These adaptors, and the revertants, as well as the parent, were sequenced.
INSTRUMENT(S): DNBSEQ-T7
ORGANISM(S): Cryptococcus neoformans var. grubii H99
Project description:Candida albicans strains SC5314 and YJB-T1891 were used as wild type to delete VMA11. The deletion strains (TJ926 and TJ927) were spread on YPD plate supplemented with 1μg/ml fluconazole. Randomly 96 adaptors derived from TJ926 and 96 adaptors derived from TJ927 were tested with disk diffusion assay. Among the resistant adaptors, randomly 15 adaptors (A4-A18) and 48 adaptors (A19-A66) derived from TJ926 were sequenced.
Project description:Candida auris clinical isolate FY279 was exposed to tebuconazole (32μg/ml). Randomly14 adaptors were chosen. 10 adaptors obtained resistance to tebuconazole. These resistant adaptors were sequenced.
Project description:Candida auris clade III isolate B12039 was spread on YPD plate supplemented with 250 ng/ml, 500 ng/ml, 1000 ng/ml, and 2000 ng/ml of caspofungin. In total, 33 adaptors were chosen for further analysis. We did sequencing of them as as well as the parent. 9 adaptors (250-1 - 250-9) were obtained from YPD+250ng/ml caspofugin. 6 adaptors (500-1 - 500-6) were obtained from YPD+250ng/ml caspofugin. 6 adaptors (1000-1 - 1000-6) were obtained from YPD+250ng/ml caspofugin. 12 adaptors (2000-1 - 2000-12) were obtained from YPD+250ng/ml caspofugin
Project description:YJB-T490 was spread on YPD-agar plates supplemented with miconazole (0.008-2ug/mL). Randomly 3 adaptors from each drug plate were sequenced. In addition, 2 miconazole adaptors derived from SC5314, and 6 adaptors derived from CDR1 deletion strain were also sequenced.
Project description:We exposed Cryptococcus neoformans lab strain H99 to amphtericin B and obtained some adaptors. We did sequencing of these adaptors.
Project description:Candida albicans lab strain SC53114 was used to obtain fluconazole-tolerant adaptors. 53 adaptors as well as the parent strain were sequenced.
Project description:Candida parapsilosis chromosome 1 trisomy strain TJ74 was exposed to 400ng/ml caspofungin. Randomly 30 adaptors were chosen. These adaptors were sequenced.
Project description:Cryptococcus neoformans lab strain H99 was spread on YPD plate supplemented with 1.5ug/ml pyraclostrobin. Randomly 23 adaptors were chosen. These adaptors were sequenced.
Project description:We used Candida albicans lab strain SC5314 to obtain tunicamycin adaptors. We did whole genome sequencing of the adaptors and the parent as well.
Project description:Cryptococcus neoformans lab strain H99 was exposed to 600ng/ml aureobasidin A. Randomly 30 adaptors were chosen. These adaptors and the wild type strains were sequenced.