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RNA sequencing of Oryza sativa japonica control and OsRALF17/19 knockout mutant in mature rice anthers

ABSTRACT: Pollen tube growth is essential for successful fertilization and stable crop yields. We constructed loss-of-function/knock-out mutants that simultaneously target two rice genes using the CRISPR/Cas9 mutagenesis system. The selected OsRALF17 and OsRALF19 genes are strongly expressed in rice bicellular/tricellular pollen and have essential functions in the pollen tube growth. For the corresponding transcriptomic analysis, we sampled mature pollen anthers from a control group and an OsRALF17/19 knock-out mutant.

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Oryza sativa Japonica Group

SUBMITTER: Eui-Jung Kim

PROVIDER: E-MTAB-12295 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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