Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Single cell RNAseq on MDA-MB-231 spheroids and in co-culture with THP1


ABSTRACT: To study cancer cells heterogeneity at the single cell level we grew cancer cells as spheroids and extracted their RNA preform SmartSeq3xpress. We grew MDA-MB-231 cells on agar coated plates for 5-10 days in DMEM 10% FBS. The spheroids were incubated for 2 hours with Calcein AM and Vybrant Dye 10uM at 37C and washed twice with PBS. After dissociation with trypsinLE 0.25% the cells were facs sorted and the fluorescence intensity for each cell was recorded. The RNA were extracted and the cDNA libraries were built according to the SmartSeq3xpress protocol.

INSTRUMENT(S): DNBSEQ-G400

ORGANISM(S): Homo sapiens

SUBMITTER: Antony Cougnoux 

PROVIDER: E-MTAB-12325 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Similar Datasets

2022-05-16 | E-MTAB-11467 | biostudies-arrayexpress
2022-05-16 | E-MTAB-11452 | biostudies-arrayexpress
2022-02-16 | E-MTAB-11433 | biostudies-arrayexpress
2022-02-16 | E-MTAB-11448 | biostudies-arrayexpress
2023-09-30 | E-MTAB-13293 | biostudies-arrayexpress
2023-11-01 | E-MTAB-12579 | biostudies-arrayexpress
2021-02-25 | E-MTAB-10148 | biostudies-arrayexpress
2011-03-28 | E-GEOD-26980 | biostudies-arrayexpress
2011-12-31 | E-GEOD-28052 | biostudies-arrayexpress
2018-04-10 | E-MTAB-6365 | biostudies-arrayexpress