Single cell RNAseq on MDA-MB-231 spheroids and in co-culture with THP1

ABSTRACT: To study cancer cells heterogeneity at the single cell level we grew cancer cells as spheroids and extracted their RNA preform SmartSeq3xpress. We grew MDA-MB-231 cells on agar coated plates for 5-10 days in DMEM 10% FBS. The spheroids were incubated for 2 hours with Calcein AM and Vybrant Dye 10uM at 37C and washed twice with PBS. After dissociation with trypsinLE 0.25% the cells were facs sorted and the fluorescence intensity for each cell was recorded. The RNA were extracted and the cDNA libraries were built according to the SmartSeq3xpress protocol.

INSTRUMENT(S): DNBSEQ-G400

ORGANISM(S): Homo sapiens

SUBMITTER: Antony Cougnoux 

PROVIDER: E-MTAB-12325 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress