3'-UTR-seq of Mus musculus female and male hypothalamus across the pubertal transition

ABSTRACT: The hypothalamus is a functionally and cellularly complex tissue controlling many developmental processes, including puberty. While key hormonal aspects of puberty regulation in the hypothalamus are well established, understanding the genes, cell-types, and epigenetic mechanisms underlying and regulating puberty is limited. Here, we performed 3’-UTR-seq on the hypothalamus from both sexes of C57BL/6J mice at 5 ages spanning pubertal transition (postnatal days 12, 22, 27, 32, 37) (4-5 replicates per sex at each age) to examine genome-wide age- and sex-biased trends in gene expression in a cell-type aware manner. Sample collection, RNA extraction, and sequencing was completed using the same protocols and on the same mice as PMID: 3622112 (E-MTAB-9459). QuantSeq 3’mRNA-seq libraries were con

INSTRUMENT(S): Illumina HiSeq 2500, Agilent NGS Workstation

ORGANISM(S): Mus musculus

SUBMITTER: Dustin Sokolowski 

PROVIDER: E-MTAB-12340 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress