Bulk RNA sequencing from sorted VChIs

ABSTRACT: VIP/ChAT colinergic interneurons were isolated from mouse cortices with the NuNeX Method described in the manuscript. Cortical neuronal networks control cognitive output, but their composition and modulation remain elusive. Here, we studied the morphological and transcriptional diversity of cortical cholinergic VIP/ChAT interneurons (VChIs), a sparse population with a largely unknown function. We focused on VChIs from the whole barrel cortex and developed a high-throughput automated reconstruction framework, termed PopRec, to characterize hundreds of VChIs from each mouse in an unbiased manner, while preserving 3D cortical coordinates in multiple cleared mouse brains, accumulating thousands of cells. We identified two fundamentally distinct morphological types of VChIs, bipolar and multipo

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Mus musculus

SUBMITTER:  

PROVIDER: E-MTAB-12395 | biostudies-arrayexpress |

SECONDARY ACCESSION(S): ERP143346

REPOSITORIES: biostudies-arrayexpress