Project description:Candida albicans lab strain SC5314 was spotted on YPD plates supplemented with 0.25-16 μg/ml tebuconazole. Randomly at least 4 papillary colonies from each plate were sequenced.
Project description:From C. albicans reference strain SC5314, CDR1, CDR2, MDR1, CMP1, CNB1, CRZ1 were deleted. The deletion strains were spread on YPD plates supplemented with tebuconazole. Randomly 4 adaptors derived from each deletion strain were sequenced.
Project description:Two tebuconazole adaptors, TJ1503 and TJ1669, were unstable. On YPD-agar plates, they gave rise to small (S) and large (L) colonies. We sequenced both types of colonies. TJ1503S and TJ1669S were the S colonies. TJ1503L and TJ1669L were the large colonies.
Project description:Candida haemulonii wild type strain TJ180 were expsoured to 64 and 128 μg/ml fluconazole. 14 resistant mutants as well as the parent were sequenced.
Project description:Candida albicans lab strain SC5314 was spread on YPD plates supplemented with ketoconazole (0.125-16μg/ml); Randomly 3 adaptors from each plate were sequenced.
Project description:Candida auris clinical isolate FY279 was exposed to tebuconazole (32μg/ml). Randomly14 adaptors were chosen. 10 adaptors obtained resistance to tebuconazole. These resistant adaptors were sequenced.
Project description:Candida parapsilosis clinical isolate #12584 was exposed to 3μg/ml and 4μg/ml amphotericin B. Randomly 30 adaptors (3 μg/ml) and 99 adaptors (4 μg/ml) were sequenced.
Project description:Candida albicans strains SC5314 and YJB-T1891 were used as wild type to delete VMA11. The deletion strains (TJ926 and TJ927) were spread on YPD plate supplemented with 1μg/ml fluconazole. Randomly 96 adaptors derived from TJ926 and 96 adaptors derived from TJ927 were tested with disk diffusion assay. Among the resistant adaptors, randomly 15 adaptors (A4-A18) and 48 adaptors (A19-A66) derived from TJ926 were sequenced.
Project description:Candida tropicalis clinical isolate #12584 was exposed to auerobasidin A (50ng/ml), Randomly 30 adaptors (T12-T41) were selected. These adaptors as well as the parent were sequenced.