Characterization of cefiderocol heteroresistance in carbapenem-resistant Acinetobacter baumannii (CRAB) cells after exposure to Human Pleural Fluid (HPF)

ABSTRACT: Objectives: Carbapenem-resistant Acinetobacter baumannii (CRAB) are one of the most difficult pathogen to treat. The only drug recently approved by the FDA that is active against CRAB is cefiderocol. However, recent studies have shown higher all-cause mortality rate in the group of patients treated with cefiderocol, that may be due to heteroresistance, a phenotype characterized by the survival of a small proportion of cells in a population seemingly isogenic. Previous studies showed that adding human fluids to CRAB cultures can lead to CFDC heteroresistance. To better understand the nature of this phenomenon, we carried out molecular and phenotypic analyses of CRAB heteroresistant bacterial subpopulations. Methods: The CRAB strain AMA40 was cultured in the presence of cefiderocol and huma

INSTRUMENT(S): NextSeq 550

ORGANISM(S): Acinetobacter baumannii

SUBMITTER: German Matias Traglia 

PROVIDER: E-MTAB-12444 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress