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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Nanopore RNA-seq of human osteosarcoma cell line U2OS treated with siRNA against MDC1, PLRG1, or control siRNA with or without pladienolide B

ABSTRACT: The purpose was to investigate transcript and splicing changes in U2OS cells following siRNA-mediated depletion of MDC1 and PLRG1 or treatment with the splicing inhibitor pladienolide B. Cells were treated with control siRNA as control condition. Nanopore sequencing of cDNA was performed after library preparation with ONT kits SQK-PCS109 and SQK-PBK004.

INSTRUMENT(S): MinION

ORGANISM(S): Homo sapiens

SUBMITTER: Sebastian Munk

PROVIDER: E-MTAB-12497 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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