Myb-domain protein Teb1 controls histone levels and centromere assembly in fission yeast

ABSTRACT: The TTAGGG motif is common to two seemingly unrelated dimensions of chromatin function M-^V the vertebrate telomere repeat and the promoter regions of many Schizosaccharomyces pombe genes, including all of those encoding canonical histones. The essential S. pombe protein Teb1 contains two Myb-like DNA binding domains related to those found in telomere proteins and binds the human telomere repeat sequence TTAGGG. Here we analyze Teb1 binding throughout the genome and the consequences of reduced Teb1 function. ChIP on chip analysis reveals robust Teb1 binding at many promoters, notably including all of those controlling canonical histone gene expression. A hypomorphic allele, teb1-1, confers reduced binding and reduced levels of histone transcripts. teb1-1 cells also show severe defects in t

ORGANISM(S): Schizosaccharomyces pombe

SUBMITTER: Samuel Marguerat 

PROVIDER: E-MTAB-1253 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress