Ricochet transcription of pachytene piRNA promoters in mammals

ABSTRACT: The origin and stability of germline noncoding RNAs remain largely uncharacterized in mammals. Here, we demonstrate that the vast majority of pachytene piRNAs originate from less than 100 bi- and unidirectionally transcribed loci in the mouse genome. These loci show features characteristic of transcribed protein-coding genes, including primary transcription by RNA polymerase II, H3K4 trimethylation marking the transcription initiation site, and H3K36 trimethylation present at elongation regions in a highly tissue-specific manner. We identified MYBL1, a spermatocyte-enriched transcription factor, as a potential driver of piRNA precursor transcripts. By similarly mapping piRNA clusters in testes of dog and opossum, we revealed that the regulatory architectures generating piRNAs appear to

ORGANISM(S): Mus musculus

SUBMITTER: Nenad Bartonicek 

PROVIDER: E-MTAB-1265 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress