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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Dual RNA-seq identifies proteins and pathways required for Clostridioides difficile colonisation

ABSTRACT: Clostridioides difficile interactions with the gut mucosa are crucial for colonisation and establishment of infection, however key infection events during the establishment of disease are still poorly defined. To better understand the initial events that occur during C. difficile colonisation, we employed a dual RNA-sequencing approach to study the host and bacterial transcriptomic profiles during C. difficile infection in a dual-environment in vitro human gut model. Temporal changes in gene expression were analysed over 3-24h post infection and comparisons were made with uninfected controls.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Clostridioides difficile

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PROVIDER: E-MTAB-12660 | biostudies-arrayexpress |

SECONDARY ACCESSION(S): ERP145092

REPOSITORIES: biostudies-arrayexpress

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Type IV pili promote early biofilm formation by Clostridium difficile

Project description:Transcriptional analysis of Clostridium difficile R20291 in biofilm formation, planktonic state and grown on blood agar RNA sequencing was performed on Clostridium difficile R20291 in three different conditions: Biofilm formation, plantonic state and grown on blood agar plates. Each condtion has 3 replicates.

2016-05-18 | E-GEOD-69001 | biostudies-arrayexpress

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Project description:Clostridium difficile infections are the leading cause of diarrhea associated to the use of antibiotics. During infection, C. difficile initiates a sporulation cycle leading to the persistence of C. difficile spores in the host and disease dissemination. Nowadays, the development of vaccine and passive immunization therapies against C. difficile have focused on toxins A and B. In the present study, we used an immunoproteome-based approach to identify immunogenic proteins located on the outer layers of C. difficile spores as potential candidates for the development of immunotherapy and/or diagnostic methods against this devastating infection. Experimental design. To identify potential immunogenic proteins on the surface of C. difficile R20291, spore coat/exosporium extracts were separated by two-dimensional electrophoresis (2-DE) and analyzed for reactivity against C. difficile spore-specific goat sera. Finally, the proteins present at the spot of the interest were in-gel digested with chymotrypsin, and the peptides generated were separated by nanoUPLC followed by MS/MS using a Quad-TOF MS, and corroborated by Ultimate 3000RS nano UHPLC coupled to QExactive Plus Orbitrap MS.

2018-07-05 | PXD008592 | Pride

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2013-08-10 | E-GEOD-45977 | biostudies-arrayexpress

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