Effect of SOPS and Cryotop vitrification of porcine blastocysts produced in vivo on their transcriptome profile

ABSTRACT: The objective of this study was therefore to evaluate the impact of simultaneous vitrification of large numbers of embryos with the OC (20 embryos per device) system on gene expression patterns of in vivo-derived blastocysts when compared to the Superfine Open Pulled Straw (SOPS) system (5-7 embryos per device) through a microarray approach. In vivo-developed embryos were surgically retrieved from 16 sows in 4 replicates. Embryos at the blastocyst stage (n=180) were selected for the experiment and divided into three experimental groups: blastocysts vitrified with the OC system (n=60), blastocysts vitrified with the SOPS system (n=60), and a control group consisting of fresh, nonvitrified blastocysts (n=60). After in vitro culture for 24 h, six pools of 8 viable embryos (n=48 embryos per gr

ORGANISM(S): Sus scrofa

SUBMITTER: Cristina Cuello 

PROVIDER: E-MTAB-12772 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress