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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Single-cell RNA sequencing data for a mixed population of K562 cells and THP-1 cells, encoded with combinatorial DNA barcodes.

ABSTRACT: Microdroplet-based co-culturing assays dissect a complex multi-cellular interactions into individual cell-cell interaction events in a highly parallelized manner. To integrate single-cell sequencing approaches into such in-droplet multicellular co-culturing assays, we demonstrate a chemistry approach for encoding the identity of droplets to their belonging cells. K562 cells and THP-1 cells were encapsulated in water-in-oil droplets, where they were labeled with DNA barcodes encoding the identity of individual droplets. Then cells were released from droplets for pooled single-cell RNA sequencing. cDNA and DNA barcodes were sequenced in separate sequencing libraries. Experiments were carried out in duplicate.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Homo sapiens

SUBMITTER: Fumiko Kawasaki

PROVIDER: E-MTAB-12819 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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