Transcriptome-wide analyses of early immune responses in lumpfish leukocytes upon stimulation with poly(I:C)

ABSTRACT: Both bacterial and viral diseases are a major threat to farmed fish, as well as wild fish. As the antiviral immune mechanisms in lumpfish (Cyclopterus lumpus L.) are poorly understood, lumpfish leukocytes were stimulated with poly(I:C), a synthetic analog of virus dsRNA, and RNA sequencing was performed. Immune genes were identified, and transcriptome-wide analyses of early immune responses in lumpfish leukocytes showed that 310 and 1872 transcripts were significantly differentially expressed 6 and 24 hours post exposure (hpe) to poly(I:C), respectively. The most enriched GO terms when time had been accounted for, were immune system processes (GO:0002376) and immune response (GO:0006955). Analysis of differentially expressed genes (DEGs) showed that among the most highly upregulated genes

INSTRUMENT(S): Illumina HiSeq 4000

ORGANISM(S): Cyclopterus lumpus

SUBMITTER: Shreesha Rao 

PROVIDER: E-MTAB-12884 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress