Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Single-cell RNA-Sequencing of neurons labeled by retrograde FastBlue tracing (SmartSeq)


ABSTRACT: Most cancers including pancreatic ductal adenocarcinoma (PDAC) are infiltrated by PNS neurons participating in their complex tumor microenvironment. However, their cell bodies and nuclei are located in the para- and pre-vertebral PNS ganglia located far from the tumor mass itself. Thus, molecular information on healthy organ- vs. cancer-infiltrating neurons is currently lacking in any sequencing dataset of healthy or tumor tissue. To specifically identify and molecularly characterize the identity and transcriptomes of PDAC-infiltrating neurons at single cell resolution, we developed “Trace-n-seq”. This method is based on retrograd tracing of axons from target tissues to their respective ganglia, followed by individual FACS-isolation and transcriptomic analysis. We characterized >2000 sympathetic and sensory neurons that infiltrate PDAC, healthy pancreas, or other abdominal organs.

INSTRUMENT(S): NextSeq 550

ORGANISM(S): Mus musculus

SUBMITTER: Jasper Panten 

PROVIDER: E-MTAB-12940 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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