Single-cell RNA and BCR sequencing of naive B cells cultured in IgG class-switching polarising conditions

ABSTRACT: Naive B cells (CD27-IgD+) were obtained from 3 healthy controls and cultured in vitro under anti-IgM, CD40L and IFN-gamma to polarise class-switching towards IgG isotypes. Single-cell RNA and BCR sequencing libraries were prepared at Day 0 (before addition of stimuli), Day 3 and Day 6 of the cell culture time-course. This is intended as a dataset to validate a new computational method sciCSR in enumerating productive and sterile immunoglobulin transcripts as indicators of B cell class switching and maturation dynamics.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Homo sapiens

SUBMITTER: Joseph Ng 

PROVIDER: E-MTAB-13050 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress