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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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CapTrap-Seq: A platform-agnostic and quantitative approach for high-fidelity full-length RNA transcript sequencing

ABSTRACT: Here we describe CapTrap-Seq, an experimental workflow designed to address the problem of reduced transcript end detection by long-read RNA sequencing methods, especially at the 5' ends. We apply CapTrap-Seq to profile transcriptomes of the human heart and brain and we compared the obtained results with other library preparation approaches. CapTrap-Seq is a platform-agnostic method and here tested the method by using 3 different long-read sequencing platforms: MinION (ONT), Sequel (PacBaio) and Sequel II (PacBio).

INSTRUMENT(S): Illumina HiSeq 2500, MinION, Sequel, Sequel II

ORGANISM(S): Homo sapiens

SUBMITTER: Sílvia Carbonell-Sala

PROVIDER: E-MTAB-13063 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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