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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of mouse MIN6 insulinoma beta cells following knock-down of murine Pdx1

ABSTRACT: In order to identify genes that are regulated by Pdx1, MIN6 insulinoma cells were transduced with adenovirus expressing a short hairpin RNA directed against murine Pdx1 (AdshPdx1) or luciferase (AdshLuc) or not transduced at all (untreated). RNA was harvested and reverse transcribed. Reduced expression of Pdx1 and established Pdx1 targets was confirmed. The RNA was then submitted for labeling and hybridization to the PancChip cDNA microarray.

ORGANISM(S): Mus musculus

SUBMITTER: David Groff

PROVIDER: E-MTAB-132 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Pdx1 (MODY4) regulates pancreatic beta cell susceptibility to ER stress.

Sachdeva Mira M MM Claiborn Kathryn C KC Khoo Cynthia C Yang Juxiang J Groff David N DN Mirmira Raghavendra G RG Stoffers Doris A DA

Proceedings of the National Academy of Sciences of the United States of America 20091023 45

Type 2 diabetes mellitus (T2DM) results from pancreatic beta cell failure in the setting of insulin resistance. Heterozygous mutations in the gene encoding the beta cell transcription factor pancreatic duodenal homeobox 1 (Pdx1) are associated with both T2DM and maturity onset diabetes of the young (MODY4), and low levels of Pdx1 accompany beta cell dysfunction in experimental models of glucotoxicity and diabetes. Here, we find that Pdx1 is required for compensatory beta cell mass expansion in r ...[more]

PMID: 19855005

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