Project description:The single-cell RNA-seq experiment aims to explore the cell-type composition of right coronary artery plaques to provide an overview of human-like atherosclerosis. The PCSK9 transgenic minipigs were subjected to 15 months of HFHC diet (HFHC group f, n=4; m, n=1). At the time of euthanasia, plaque parts without media were quickly processed and analyzed by scRNA-sequencing.

2025-08-30 | E-MTAB-15464 | biostudies-arrayexpress

scRNA-seq for the monkey primary visual cortex

Project description:Transcriptomes of monkey primary visual cortex at the single-cell resolution. The dataset includes all cell types, including both glia and neurons.

2022-10-24 | E-MTAB-10459 | biostudies-arrayexpress

Single cell RNA-seq of human breast skin cells

Project description:Cells were generated for a single cell atlas of adult human skin to dissect the cellular and molecular organisation underpinning human skin immune barrier function. Surplus skin from breast reconstruction surgery was collected (n=3), then the top 200 μm layer was taken. Epidermis was separated from the dermis, and both layers were separately digested. Single cells were FACS sorted into eight categories, then loaded onto a 10x Genomics Chromium Controller, and sequenced on an Illumina HiSeq 4000.

2021-02-10 | E-MTAB-8142 | biostudies-arrayexpress

Single cell CITE-seq and VDJ sequencing of epidermal and blood T cells

Project description:The aim of the experiment was to investigate the clonal relationship between CD8+ T cell subsets of donor matched blood with epidermal CD8+ T cells. Abdominal skin (n=8) was separated into dermis and epidermis, followed by epidermal skin and PBMC isolation. Single CD8+ T cells (n=6) and CD3+ T cells (n=2) were FACS sorted, processed with the 5’ 10x Genomics workflow and sequenced at NGI Sweden.

2023-06-12 | E-MTAB-12839 | biostudies-arrayexpress

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