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E2F4-p53 Re-ChIP seq in HCT116 wild-type cells

ABSTRACT: DREAM complex is known to regulate DNA repair and cell cycle-regulated genes in a p53-p21 dependent axis. This experiment identified an alternate pathway of DREAM target regulation.

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Homo sapiens

SUBMITTER: Sagar Sengupta

PROVIDER: E-MTAB-13359 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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E2F4-p53 Re-ChIP seq in HCT116 p21-/- cells & HCT116 p53-/- TAD mutant cells

Project description:DREAM complex is known to regulate DNA repair and cell cycle-regulated genes in a p53-p21 dependent axis. This experiment identified an alternate pathway of DREAM target regulation.

2025-08-23 | E-MTAB-14629 | biostudies-arrayexpress

E2F4-p53 Re-ChIP seq in HCT116 wild-type cells

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Expression profile of HCT116 p53+/+ and P53-/- cells treated with Leptomycin B

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Expression signature in a human colorectal cancer cell line HCT116 transfected with miR-1 precursor molecule

Project description:We identified that miR-1 is silenced in association with CpG island hypermethylation in a colorectal cancer (CRC) cell line, HCT116. To determine whether miR-1 serves as a tumor suppressor in CRC, we transfected CRC cell lines with a miR-1 precursor molecule or a negative control, and carried then out a series of MTT assays. Forty-eight hours after transfection, we observed that ectopic expression of miR-1 moderately suppressed growth in all three cell lines. To further clarify the effect of the miRNA, we next performed gene expression microarray analysis in HCT116 cells transfected with a miR-1 precursor molecule or a negative control. We found that 2769 probe sets were downregulated (> 1.5-fold) by ectopic miR-1 expression, and gene ontology analysis revealed that “extracellular regions”, “membrane” and “response to wound healing” genes were significantly enriched among the downregulated genes. HCT116 cells were transfected with a Pre-miR-1 miRNA Precursor Molecule (Ambion) or Pre-miR miRNA Molecules Negative Control #1 (Ambion). Forty-eight hours after transfection, total RNA extraction were carried out, and gene expression signatures were analyzed.

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