Gene expression of erythromyeloid K-562 cells

ABSTRACT: K-562 cells were cultured in standard growth conditions and used to produce five distinct cell cultures: wild type, FHC-silenced,wild type hemin-treated, FHC-silenced hemin-treated. Then the cells were lysed and RNA was extracted using TRIzol method.Total RNA were fluorescently labelled, amplified and hybridized in triplicate for 18 hours on Illumina HumanHT12 v.4.0 Expression BeadChips and after scanning, analysis was performed with GenomeStudio v.2010.3 software, for quality control and mRNA expression analysis.

ORGANISM(S): Homo sapiens

SUBMITTER: Concetta Faniello 

PROVIDER: E-MTAB-1336 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress